RNA Interference RNAi Information, Procedures and Protocols to help you purify oligo RNAs, anneal and store siRNA, siRNA design tools and information, and cloning of small hairpin RNA.
| Bacteria-Mediated (Feeding) RNAi Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Feeding_RNAi.htm Bacteria-Mediated (Feeding) RNAi Protocol. This protocol is based on: Timmons L, Court DL, Fire. Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene. 2001 Jan 24;263(1-2):103-12. - [Read more] |
| Cloning siRNA Duplexes into pSUPER or pSUPERretro Vectors - http://www.flemingtonlab.com/Protocols/pSUPERbasedsiRNAs.pdf Cloning siRNA Duplexes into pSUPER or pSUPERretro Vectors - [Read more] |
| Drosophila RNAi Screening Center - http://flyrnai.org/RNAi_FAQ_rnai_on_cc.html Drosophila RNAi Screening Center - protocols listed here are for Drosophila cells in 6 well plates and our pre-aliquoted 384 well plates. RNAi experiments may be done in other size plates, just scale up or down. - [Read more] |
| Lentiviral RNAi Protocols - http://www.sciencegateway.org/protocols/lentivirus/index.htm Lentiviral RNAi Protocols - Long DNA oligos containing the target sequence are cloned into LentiLox 3.7 (see protocol). - [Read more] |
| Protocol for siRNA Annealing and Storage - http://www.eurogentec.com/module/FileLib/PROTOCOL-SIRNA-0702-V2.pdf Protocol for siRNA Annealing and Storage. - [Read more] |
| RNA interference protocol - http://www.mann.embl-heidelberg.de/GroupPages/PageLink/activities/iTAP/Experimental/RNAi.html RNA interference protocol - [Read more] |
| RNA Interference Resource - http://www.ambion.com/techlib/resources/RNAi/ RNA Interference Resource Site - [Read more] |
| RNAi target selection rules: - http://www.rnaiweb.com/RNAi/siRNA_Design/index.html RNAi target selection rules: - [Read more] |
| Selection of siRNA duplexes from the target mRNA sequence - http://www.rockefeller.edu/labheads/tuschl/sirna.html Selection of siRNA duplexes from the target mRNA sequence - [Read more] |
| siRNA Design Guidelines - http://www.ambion.com/techlib/tb/tb_506.html siRNA Design Guidelines - [Read more] |
| Upstate RNA Interference RNAi Troubleshooting Site - http://www.upstate.com/misc/protocol_detail.q.prot.e.siRNA-troble-tips.a.name.e.siRNA_Troubleshooting_Tips Upstate RNA Interference RNAi Troubleshooting Site - [Read more] |
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