Acetate Precipitation DNA Isolation - http://www.genome.ou.edu/DblAcetateProcV3.html Acetate Precipitation DNA Isolation Protocol through a Clear Lysate. - [Read more]

Cloning Review - http://opa.faseb.org/pdf/cloning.pdf Cloning Review Paper. Great. Discusses the past, present and the future on cloning. - [Read more]

Cloning Tutorial - http://www.macvector.com/Resources/ClickCloningTutorial.pdf Cloning Tutorial, great for learning cloning and the cloning procedure. - [Read more]

Cloning Vector Basics - http://jbpc.mbl.edu/GenomesCourse/media/200510171900-vectors.pdf Cloning Vector Basics, for genomic, cDNA and subcloning. - [Read more]

DNA Cloning Course - http://www.sabc.murdoch.edu.au/courses/DNABrochure_web.pdf DNA Cloning Course from murdochuniversity. - [Read more]

Molecular Cloning - http://www.fpl.fs.fed.us/documnts/pdf1989/schal89a.pdf Molecular Cloning, Basic Method Article. - [Read more]

Physicla Mapping Cloning - http://www.apsnet.org/mpmi/PDFS/1997/1014-01N.pdf Physicla Mapping through Cloning. - [Read more]

Quick Shotgun Cloning of Phage Inserts - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/molecularCloning/phageInsertSubcloning.html Quick Shotgun Cloning of Phage Inserts Protocol and Information Links from the University of Zurich. - [Read more]

Random subclone generation - http://www.genome.ou.edu/protocol_book/protocol_partII.html#II Random subclone generation protocol with information and directions on sonication, nebulization, Random fragment end-repair, size selection, and phosphorylation, DNA ligation, Competent cell preparation, Calcium Chloride treated bacterial cell transformation, and Microcentrifuge Tube Transformation. - [Read more]

Recombinant DNA and Cloning - http://biochem.med.ufl.edu/images/MolBiol04/Lecture%2054-2.pdf Recombinant DNA and Cloning lecture. - [Read more]

Subcloning Procedures - http://www.its.caltech.edu/%7Ebjorker/Martin_subcloning%20procd.pdf Extensive 7 page PDF protocol on all cloning procedures and techniques. Great link ! - [Read more]

Subcloning Tips from the Experts - http://www.its.caltech.edu/%7Ebjorker/PJB%27s_cloning_hints.pdf Expert tips for cloning. - [Read more]

Subtractive Cloning - http://web.wi.mit.edu/sive/pub/Lab%20Resources%20Documents/Sagerstrom%20SubCloning.pdf Subtractive cloning is a powerful technique that allows isolation of the differences in the nucleic acid composition of two cell samples. This is a paper on subtractive cloning from Annu. Rev. Biochem. 1997. - [Read more]

Vector systems for cloning different sizes of DNA fragments - http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg.section.406 Cell-based DNA cloning has been used widely as a tool for producing quantities of pure DNA for physical characterization and functional studies of individual genes, gene clusters or other DNA sequences of interest. However, the size of different DNA sequences of interest can vary enormously (e.g. human gene sizes are known to vary between 0.1 kb and 2.5 Mb). - [Read more]